Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection
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چکیده
منابع مشابه
Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection
BACKGROUND Methodologies like phage display selection, in vitro mutagenesis and the determination of allelic expression differences include steps where large numbers of clones need to be compared and characterised. In the current study we show that high-resolution melt curve analysis (HRMA) is a simple, cost-saving tool to quickly study clonal variation without prior nucleotide sequence knowled...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2009
ISSN: 1472-6750
DOI: 10.1186/1472-6750-9-50